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. 2016 Oct 3;7:13056. doi: 10.1038/ncomms13056

Figure 2. Construction of logic AND circuits by integrating split dCas9 domains.

Figure 2

(a) Schematic representation of a three-input logic AND circuit by using two split dCas9 fragments (dCas9N:IntN and IntC:dCas9C:Suntag) and the rapalog that promote the dimerization of ScFv:GB1:FKBP and FRB*:VP64. The dCas9 constituents are split at residue 1153. (b) Schematic representation of reconstitution of three split-dCas9 fragments. The dCas9 constituents are split at residue 713 and 1153. (a,b) Data are shown as the mean fold change (mean±s.e.m.; n=3) of TagBFP fluorescence measured by using flow cytometer 48 h after transfection into HEK293 cells.