Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 3;7:13056. doi: 10.1038/ncomms13056

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

(a) Schematic representation of a two-input and two-output sensory switch by swapping split dCas9 domains that recognize two different PAM sequences. The dCas9 constituents are split at residue 713. The light blue rectangle represents the mutant dCas9 domain (VRER) that can recognize the NGCG PAM sequences but not the NGG PAM sequences. The ModTRE2 promoter contains seven repeats of gRNAb binding sites with the NGCG PAM sequences upstream of a miniCMV core. (b) Setting states of the sensory switch by artificial shRNA-FF5 and shRNA-FF4. Representative scatter plots and microscopic images are shown in the right panel. Each red scale bar in images represents 50 μm. (c) The response of the sensory switch to varying amount of shRNA-FF4. The solid line was plotted by qplot function in R package. (b,c) Each bar or data point shows mean fold changes (mean±s.e.m.; n=3) of EYFP or mKate2 measured by using flow cytometer 48 h after transfection.