Figure 1. ngs1 (orf19.7516) and ngt1 are specifically defective in GlcNAc signalling and catabolism.

(a) Cells of wild-type (SN250), orf19.7516, snf4, nag1 and ngt1 (HLY4394) mutant strains were serially diluted 10-fold and spotted onto YNB solid medium containing 2.5 mM of the indicated sugar or SC solid medium containing 2.5 mM GlcNAc. The YNB medium was supplemented with 0.1 mg ml⁻¹ arginine to permit growth. Photographs were taken after 2 days of growth at 30 °C. (b) qRT-PCR analysis of GlcNAc catabolic genes DAC1 and HXK1 and GlcNAc transporter NGT1 upon GlcNAc induction in the wild type (SN250) and indicated mutants. Cells were grown in liquid SC medium with 2.5 mM GlcNAc (n) or 5% glycerol (g) for 2 h at 30 °C for RNA extraction. Mean data±s.d. from three independent qRT-PCR experiments was plotted.