TABLE 1.

Kinetic parameters for GspDAH7PS^WT, GspDAH7PS^D, and GspDAH7PS^C

Protein	DAH7PS activitya					CM activity
	KmPEP	KmE4P	kcat	kcat/KmPEP	kcat/KmE4P	Km	kcat	kcat/Km
	μm	μm	s−1	mm−1s−1	mm−1s−1	μm	s−1	mm−1s−1
GspDAH7PSWT	87 ± 9	95 ± 8	45 ± 4	0.52	0.47	88 ± 6	1.9 ± 0.1	2.1 × 10−2
GspDAH7PSD	98 ± 11	62 ± 5	63 ± 5	0.64	1.0
GspDAH7PSC						450 ± 40	5.5 ± 0.5	1.2 × 10−2

^a The Michaelis constants for the DAH7PS activity are apparent values determined with a fixed concentration of the invariant substrate (as described under “Experimental Procedures”).