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. 2016 Aug 8;291(42):21836–21847. doi: 10.1074/jbc.M116.741637

TABLE 2.

Primers for generation of constructs

GspDAH7PSC primers (round 1)
    Forward primer 5′-GGCAGCGGCGCGATGGGTAATGAACGTCTG
    Reverse primer 5′-GAAAGCTGGGTGTTATTTACGATGATCATCTTC

GspDAH7PSD primers (round 1)
    Forward primer 5′-GGCAGCGGCGCGATGGCACTGCTGGTTAGC
    Reverse primer 5′-GAAAGCTGGGTGTTACTCGAGTGCACGAAC

Generic primers (round 2)
    Forward primer 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTCGAAAA CCTGTATTTTCAGGGCAGCGGCGCG
    Reverse primer 5′-GGGGACCACTTTGTACAAGAAAGCTGGT