FIGURE 5.

RAMP-CLR responses appear PTX-insensitive when assayed using HEK-293S cells. cAMP accumulation was determined in the presence (treated) and absence (untreated) of PTX from HEK-293S cells transiently transfected with CLR and RAMP1 (n = 5) (A), RAMP2 (n = 5) (B), and RAMP3 (n = 5) (C). Cells were stimulated for 30 min with CGRP, and AM2. Data are expressed as percentage of the maximal cAMP production as determined using 100 μm forskolin stimulation in the presence of PTX and are means ± S.E. of n individual data sets.