FIGURE 1.

Schematic of PKCα peptide FRET sensors. PKCα peptide sensors are based around an ER/K FRET linker (14, 15), which separates the catalytic domain of PKCα and a variety of short peptides ≤14 residues in length (Fig. 2). In the majority of the experiments, the fluorophores used in the ER/K linker are monomeric Cerulean (mCer, FRET donor) and monomeric Citrine (mCit, FRET acceptor). For the time-resolved measurements in Fig. 4A, monomeric eGFP and monomeric Cherry (mCherry) were used instead.