FIGURE 4.

Leptin promotes proliferation of TH2 cells and ILC2s. A, intracellular stain of Ki67 in LLN TH2 cells and ILC2s from WT and Ob^−/− mice after induction of experimental asthma. Bar graphs depict means and S.D. in each group mice (n = 5–6 per group); Student's t test; *, p ≤ 0.05 and **, p ≤ 0.005. B, flow cytometry of CFSE dilution in TH2 cells during in vitro differentiation with leptin at indicated concentrations. C, flow cytometry of CFSE dilution in TH2 cells during 1-day restimulation with plate-bound anti-CD3 in the presence of leptin at indicated concentrations. Data shown are representative of two (A) or three (B, C) experiments.