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Leptin protects TH2 cells from activation induced cell death. A–C, cells were cultured in the absence or presence of leptin at indicated concentrations. A, flow cytometry of activation induced cell death in TH2 cells after restimulation with plate-bound anti-CD3 for 24 h. B, intracellular stain of Bcl-2 in in vitro polarized TH2 cells. C, immunoblot of Bcl-2 expression in polarized TH2 cells. Data shown are a representative of two (A, C) or three (B) experiments.
