TABLE 2.
Water consumption, renal parameters, and circulating vasopressin in wild-type and VAMP3−/− mice
Blood and 24-h urine samples were collected to measure volume, osmolality, urinary Na+ (UNa+), urinary K+ (UK+), urinary Cl− (UCl−), urinary Ca2+ (UCa2+), urinary Mg2+ (UMg2+), urinary creatinine (UCreatinine) excretion, and serum vasopressin. The measurements were performed under baseline conditions and after 24-h water deprivation. The values are the means ± S.E. The p values for comparisons between wild type and VAMP3−/− are indicated (n = 6).
| Baseline |
24-h water deprivation |
|||||
|---|---|---|---|---|---|---|
| Wild type | VAMP3−/− | p value | Wild type | VAMP3−/− | p value | |
| Water consumed (ml/24 h) | 4.7 ± 0.2 | 5.1 ± 0.3 | 0.35 | |||
| Urine volume (μl/24 h) | 1337 ± 118 | 1625 ± 167 | 0.19 | 650 ± 85 | 673 ± 85 | 0.86 |
| Urine osmolality (mOsm) | 3433 ± 166 | 2924 ± 131 | 0.04a | 5307 ± 235 | 5763 ± 456 | 0.40 |
| UNa+ (μmol/day) | 264.8 ± 18.9 | 266.3 ± 31.2 | 0.97 | 445.3 ± 33.2 | 591.4 ± 53.8 | 0.04a |
| UK+ (μmol/day) | 779.8 ± 51.2 | 781.9 ± 98.2 | 0.99 | 1100.0 ± 77.8 | 1491.3 ± 105.8 | 0.01a |
| UCl− (μmol/day) | 541.4 ± 43.7 | 611.5 ± 65.8 | 0.40 | 823.7 ± 57.0 | 1137.9 ± 92.9 | 0.02a |
| UCa2+ (μmol/day) | 0.60 ± 0.02 | 0.81 ± 0.05 | 0.01a | 1.06 ± 0.12 | 1.38 ± 0.12 | 0.08 |
| UMg2+ (μmol/day) | 1.90 ± 0.19 | 1.73 ± 0.13 | 0.50 | 5.47 ± 0.62 | 5.16 ± 0.58 | 0.73 |
| UCreatinine (mg/day) | 0.31 ± 0.02 | 0.35 ± 0.04 | 0.37 | 0.89 ± 0.09 | 1.17 ± 0.12 | 0.09 |
| Vasopressin (pg/ml) | 5.7 ± 1.4 | 4.6 ± 1.7 | 0.64 | 41.9 ± 8.5 | 64.4 ± 12.1 | 0.16 |
a p < 0.05.