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. 2016 Aug 17;291(42):22106–22117. doi: 10.1074/jbc.M116.731695

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© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.

Author's Choice—Final version free via Creative Commons CC-BY license.

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FIGURE 10. — CgT and the helix domain are required for dGT1 function in vivo. Wild type S. parasanguinis FW213 (lane 1), Fap1 mutant (lane 2), dGT1 mutant (lane 3) and its complement variants, dGT1 complemented with the full-length dGT1 (lane 4), DUF1792 (lane 5), CgT (lane 6) with dGT1 without the helix domain (lane 7), and with both DUF1792 and CgT (lane 8) were examined for the production of Fap1 by Western blotting analysis using Fap1 peptide-specific antibody E42 and mature Fap1-specific antibody F51.