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Gel filtration profile of CgT and CgTΔH1–3 (A) and pulldown assay (B). Elution curve of CgT (blue) and CgTΔH1–3(red) from a GE Hiload 16/60 Superdex G200 column at 4 °C. B, GST pull-down of His-tagged CgT. GST fused dGT1 (lane 1), DUF1792 (lane 2), and CgT (lane 3) were used to pull down His-tagged CgT. Top panel, His-CgT pulled down by GST fusion proteins, probed by anti-His antibody; bottom panel, input control of His-tagged CgT, probed by anti-His antibody.
