TABLE 2.
ITC thermodynamic parameters
ΔH, ΔS, number of binding sites (n), and the binding constant (Kd) for donor binding with proteins in solution at 20 °C.
Cell | Ligand | Number of sites | Kd | ΔH | ΔS | |
---|---|---|---|---|---|---|
n | mm | cal/mol | cal/mol/deg | |||
A | CgT | UDP-GlcNAc | 1.10 ± 0.02 | 10.67 ± 1.33 | −4358.5 ± 65.5 | 3.80 ± 0.47 |
B | CgTΔH1–3 | UDP-GlcNAc | 0.88 ± 0.01 | 12.15 ± 1.35 | −2148 ± 63 | 11.45 ± 0.45 |