TABLE 1.
Candidate LRP1-resistant mutants of TIMP-3 generated
Using a model of TIMP-3, 10 pairs of Lys residues were predicted to be separated by 21 ± 5 Å on the surface of TIMP-3, and 10 double Lys → Ala mutants were generated. The 12 constituent single Lys → Ala mutants generated are also listed.
| Mutant | Distance between lysine α-carbon atoms |
|---|---|
| Å | |
| Double Lys → Ala mutants of TIMP-3 generated | |
| K22A/K110A | 16.7 |
| K22A/K157A | 22.3 |
| K26A/K45A | 19.4 |
| K27A/K45A | 23.9 |
| K30A/K52A | 23.0 |
| K30A/K76A | 21.2 |
| K42A/K110A | 20.6 |
| K42A/K157A | 25.9 |
| K45A/K89A | 15.6 |
| K71A/K157A | 24.6 |
| Single Lys → Ala mutants of TIMP-3 generated | |
| K22A | |
| K26A | |
| K27A | |
| K30A | |
| K42A | |
| K45A | |
| K52A | |
| K71A | |
| K76A | |
| K89A | |
| K110A | |
| K157A | |