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. 2016 Sep 1;291(42):22218–22230. doi: 10.1074/jbc.M116.740563

FIGURE 4.

FIGURE 4.

Duplex DNA release upon removal of ATP. A, plot showing the normalized RPA-eGFP signal intensity when preassembled RecA presynaptic complexes are chased with buffer containing no nucleotide cofactor. The increased signal intensity reflects dissociation of unlabeled RecA from the ssDNA and its replacement by RPA-eGFP. B, survival probability plot for the 70-bp dsDNA harboring a single 8-bp microhomology insert when chased with buffer containing either 1 mm ATP or no nucleotide cofactor. C, survival probability plot for the 70-bp dsDNA harboring a single 15-bp microhomology insert when chased with buffer containing either 1 mm ATP or no nucleotide cofactor.