FIGURE 4.

VPA down-regulates expression of nutrient transporters via ceramide. mRNA levels of nutrient transporters were quantified by qRT-PCR in WT (A) and rsb1Δ (B) cells grown in the presence or absence of VPA and fumonisin, as indicated. Values are reported as -fold change in expression in cells grown in VPA relative to cells grown without VPA. Expression was normalized to the mRNA levels of the internal control ACT1. Data shown are mean ± S.D. (error bars) (n = 6) (*, p < 0.05; **, p < 0.01).