FIGURE 7.
Vtc5 expression changes the localization of the transcription factor Pho4. A, indicated mutants had been transformed with a centromeric plasmid encoding Pho4 under the control of its own promoter. The localization of Pho4-GFP was assessed in living cells cultured in SC medium (SC), after 15 min of Pi starvation (SC-Pi), and 15 min after re-addition of 10 mm Pi to Pi-starved cells (SC-Pi, + Pi). Scale bar, 5 μm. B–D, percentage of cells showing enrichment in nuclear Pho4 was determined for these conditions. At least 200 cells were counted per condition. n = 3. ***, p < 0.0001; **, p < 0.05.