FIGURE 3.

Acetylation inhibits and deacetylation enhances the MbtA enzyme activity. A, time-dependent inactivation of Msmeg MbtA by acetylation. Msmeg MbtA activity was monitored at different time intervals with the additional components: ●, cAMP, Msmeg Pat and acetyl-CoA; ▴, cAMP and acetyl-CoA; ▵, cAMP and Msmeg Pat. B, time-dependent reactivation of acetylated Mtb MbtA by deacetylation. After acetylation by Msmeg Pat, Mtb MbtA was then incubated with the following components: ●, NAD⁺ and Mtb DAc; ▴, Mtb DAc; ○, NAD⁺.