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Construction and verification of Mtb Pat (Rv0998) and Mtb DAc (Rv1151c) deletion mutants. A and C, Rv0998 and Rv1151c adjacent gene organizations are shown. B and D, the ORF removal in ΔRv0998 and ΔRv1151c deletion strains are replaced by sacB-hygR cassette. E and F, left and right flanking PCR were used to confirm deletion of Rv0998 and Rv1151c with specific set of primers.
