FIGURE 8.

Luciferase assay measuring decreased gene expression under rluF gene knock-out background when the coding sequence is preceded by multiple tyrosine codons. A recombinant firefly luciferase gene was constructed with a set of 10 tyrosine codons ((TAY)₁₀-FF) or 10 asparagine codons ((AAY)₁₀-FF) preceding the firefly luciferase open reading frame. Expression of the luciferase gene in combination with either Renilla luciferase or RluF gene in the rluF gene knock-out background was conducted using a pETDuet vector. The type of recombinant construct used in the luciferase assay is denoted on the x axis. At least four to five biological replicates were tested for each construct. Error bars represent S.D. of replicate measurements.