Figure 2. The phenotypic changes after inducible ectopic expression of TLK2 in the MCF10A benign breast epithelial cells.

(a) Bar chart showing TLK2 gene expression in a panel of breast cancer and benign cell lines based on exon expression array data²¹. (b) Western blot detecting the TLK2 proteins ectopically expressed in MCF10A cells after induction with different doses of doxycycline (Dox). (c) Cell survival following induction of TLK2 expression in MCF10A cells was measured by clonogenic assay. Error bars represent the s.d. of three replicate measurements per condition. (d) Transwell migration and matrigel invasion assays. After 2-week induction of TLK2 expression with different doses of Dox, Dox was either continued or withdrawn to test if the increased cell migration and invasion is dependent on TLK2 expression. Error bars represent the s.d. of three replicate measurements per condition.