Figure 1.

Embryonic liver fordin expression is upregulated in fibrotic livers and hepatic stellate cells. A: The Embryonic liver fordin (ELF) expression in cirrhotic livers was determined by the immunohistochemical analysis. Magnification × 200; B: Real-time RT-PCR and Western blot analysis were used to evaluate the ELF expression in the liver homogenates from the control and CCl₄-treated mice. ^aP < 0.05, the CCl₄-treated mice vs the control mice. GAPDH was used as the control; C: Real-time RT-PCR and Western blot analysis were used to evaluate the ELF expression in the primary hepatic stellate cells (HSCs) isolated from the control and CCl -treated mice. ^bP < 0.01, the CCl₄-treated mice vs the control mice; D: The α-SMA and collagen I expression at the protein level were upregulated in the whole liver homogenates from the CCl₄-treated mice compared with the controls.