Figure 5.

Non-targeted profiling of indolic metabolites upon B. cinerea infection in non-mycorrhizal and mycorrhizal plants either normally fertilized or under a transient N depletion. Non-mycorrhizal infected tomato plants (BB) and mycorrhizal infected tomato plants (BBAM) with (−N) or without 48 h of nitrogen depletion and fungal inoculation at 72 hpi were processed for relative quantification by HPLC-QTOF MS. The concentration of the metabolites was determined in all samples by normalizing the chromatographic area for each compound against the dry weight of the corresponding sample. All metabolites were precisely identified using chemical standards, except for N-acetyl-5metoxytriptamine, which was identified by its fragmentation spectrum. Leaf material from 3 individual plants was pooled for each treatment combination. Box plots represent the means for three independent experiments with two technical replicates. Dotted arrows indicate multiple metabolic steps; solid arrows indicate single steps. Different letters indicate statistically significant differences (ANOVA, Fisher's Least Significant Difference (LSD) test; P < 0.05, n = 6).