Fig. 4. Immunohistochemistry revealed that expression of Wnt3a, β-catenin, vascular endothelial growth factor (VEGF), and Wnt10b all increased in both the 0.25 mm/10 cycles and 0.5 mm/10 cycles groups compared with the control group, especially in the epidermis and hair follicle epithelium. Wnt10b showed marked increased expression in the hair follicle epithelium in the 0.5 mm/10 cycles group compared with the control group (×200).