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. 2016 Sep 30;28(5):586–592. doi: 10.5021/ad.2016.28.5.586

Table 1. Primers used for polymerase chain reaction amplications.

Primer name Forward Reverse Melting temperature (℃)
Wnt3a CCGGACTTCCGCACCATCGG GGGTCTCCACCCAGCCACGA 65
β-catenin GGCAGCGGCAGGATACACGG CAGGACACGAGCTGACGCGG 60.6
VEGF TCTTCAAGCCATCCTGTGTG GCGAGTCTGTGTTTTTGCAG 56
Wnt10b CCCGGGACATCCAGGCGAGA CTCTGGCGCTGCCCTCCAAC 65
GAPDH CCCCAGCAAGGACACTGAGCAA GGCTCCCTAGGCCCCTCCTGTTAT 60

VEGF: vascular endothelial growth factor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.