Table 1. Primers used for polymerase chain reaction amplications.
Primer name | Forward | Reverse | Melting temperature (℃) |
---|---|---|---|
Wnt3a | CCGGACTTCCGCACCATCGG | GGGTCTCCACCCAGCCACGA | 65 |
β-catenin | GGCAGCGGCAGGATACACGG | CAGGACACGAGCTGACGCGG | 60.6 |
VEGF | TCTTCAAGCCATCCTGTGTG | GCGAGTCTGTGTTTTTGCAG | 56 |
Wnt10b | CCCGGGACATCCAGGCGAGA | CTCTGGCGCTGCCCTCCAAC | 65 |
GAPDH | CCCCAGCAAGGACACTGAGCAA | GGCTCCCTAGGCCCCTCCTGTTAT | 60 |
VEGF: vascular endothelial growth factor, GAPDH: glyceraldehyde 3-phosphate dehydrogenase.