Table 1.
mRNA levels of T3SS genes in the higA::Tn mutant compared to those in wild type PA14.
| Locus Tag PA14 | Locus Tag PAO1 | Name | Product | Fold changes higA::Tn/WT (E) | P-value | Fold changes higA::Tn/WT (S) | P-value |
|---|---|---|---|---|---|---|---|
| PA14_RS17315 | PA1690 | pscU | Translocation protein in type III secretion | 5.040 | 5.03E-18 | 3.733 | 1.51E-12 |
| PA14_RS17310 | PA1691 | pscT | Translocation protein in type III secretion | 6.569 | 5.63E-16 | 3.901 | 2.57E-08 |
| PA14_RS17305 | PA1692 | Probable translocation protein in type III secretion | 4.022 | 3.45E-05 | 5.280 | 4.73E-06 | |
| PA14_RS17300 | PA1693 | pscR | Translocation protein in type III secretion | 4.259 | 2.16E-13 | 4.456 | 6.79E-11 |
| PA14_RS17295 | PA1694 | pscQ | Translocation protein in type III secretion | 4.940 | 9.64E-21 | 5.341 | 1.30E-21 |
| PA14_RS17285 | PA1696 | pscO | Translocation protein in type III secretion | 6.773 | 4.84E-13 | 7.603 | 1.83E-16 |
| PA14_RS17275 | PA1698 | popN | Type III secretion outer membrane protein PopN precursor | 9.668 | 1.99E-17 | 10.303 | 2.25E-19 |
| PA14_RS17270 | PA1699 | Pcr1 | 7.646 | 6.87E-22 | 10.891 | 3.05E-29 | |
| PA14_RS17265 | PA1700 | Pcr2 | 9.520 | 3.65E-12 | 8.711 | 1.06E-11 | |
| PA14_RS17260 | PA1701 | Pcr3 | 15.069 | 3.35E-26 | 4.898 | 6.08E-11 | |
| PA14_RS17255 | PA1702 | Pcr4 | 7.637 | 1.40E-07 | 3.461 | 5.32E-03 | |
| PA14_RS17250 | PA1703 | pcrD | Type III secretory apparatus protein PcrD | 8.296 | 2.55E-36 | 4.419 | 8.01E-21 |
| PA14_RS17245 | PA1704 | pcrR | Transcriptional regulator protein PcrR | 10.180 | 1.10E-07 | 3.673 | 1.04E-03 |
| PA14_RS17190 | PA1715 | pscB | Type III export apparatus protein | 10.823 | 2.91E-27 | 7.750 | 1.25E-22 |
| PA14_RS17185 | PA1716 | pscC | Type III secretion outer membrane protein PscC precursor | 7.545 | 8.13E-34 | 5.668 | 5.06E-26 |
| PA14_RS17180 | PA1717 | pscD | Type III export protein PscD | 8.176 | 8.09E-17 | 6.118 | 5.71E-14 |
| PA14_RS17175 | PA1718 | pscE | Type III export protein PscE | 3.671 | 3.44E-10 | 5.666 | 1.50E-18 |
| PA14_RS17170 | PA1719 | pscF | Type III export protein PscF | 3.486 | 4.18E-07 | 6.825 | 4.20E-14 |
| PA14_RS17165 | PA1720 | pscG | Type III export protein PscG | 4.787 | 3.27E-17 | 6.694 | 4.94E-24 |
| PA14_RS17160 | PA1721 | pscH | Type III export protein PscH | 5.776 | 2.08E-19 | 5.984 | 2.64E-19 |
| PA14_RS17155 | PA1722 | pscI | Type III export protein PscI | 5.717 | 5.44E-22 | 4.898 | 2.78E-19 |
| PA14_RS17150 | PA1723 | pscJ | Type III export protein PscJ | 6.078 | 7.63E-27 | 5.100 | 1.98E-22 |
| PA14_RS17145 | PA1724 | pscK | Type III export protein PscK | 10.686 | 3.18E-32 | 5.262 | 2.00E-14 |
| PA14_RS17140 | PA1725 | pscL | Type III export protein PscL | 7.367 | 4.46E-30 | 5.816 | 2.25E-22 |
| PA14_RS17240 | PA1705 | pcrG | Regulator in type III secretion | 10.210 | 4.38E-09 | 7.620 | 4.03E-08 |
| PA14_RS17235 | PA1706 | pcrV | Type III secretion protein PcrV | 6.118 | 4.35E-30 | 9.030 | 5.90E-44 |
| PA14_RS17230 | PA1707 | pcrH | Regulatory protein PcrH | 6.341 | 9.18E-29 | 19.662 | 1.89E-73 |
| PA14_RS17225 | PA1708 | popB | Translocator protein PopB | 5.914 | 1.39E-21 | 10.593 | 1.10E-37 |
| PA14_RS17220 | PA1709 | popD | Translocator outer membrane protein PopD precursor | 4.516 | 3.14E-12 | 6.590 | 5.54E-20 |
| PA14_RS17215 | PA1710 | exsC | ExsC, exoenzyme S synthesis protein C precursor. | 4.807 | 1.56E-23 | 6.655 | 3.21E-34 |
| PA14_RS17210 | PA1711 | ExsE | 4.700 | 2.32E-05 | 5.277 | 1.48E-06 | |
| PA14_RS17205 | PA1712 | exsB | Exoenzyme S synthesis protein B | 5.985 | 1.56E-21 | 5.144 | 5.97E-22 |
| PA14_RS17200 | PA1713 | exsA | Transcriptional regulator ExsA | 5.374 | 4.24E-24 | 4.824 | 4.09E-22 |
| PA14_RS17195 | PA1714 | ExsD | 6.886 | 2.26E-16 | 9.658 | 9.68E-24 | |
| PA14_RS00230 | PA0044 | exoT | Exoenzyme T | 6.141 | 4.01E-28 | 8.190 | 3.39E-41 |
| PA14_RS14785 | PA2191 | exoY | Adenylate cyclase ExoY | 6.589 | 1.35E-30 | 6.053 | 1.75E-31 |
| PA14_RS20960 | exoU | ExoU | 4.443 | 1.56E-21 | 8.603 | 1.97E-44 | |
| PA14_RS20955 | spcU | SpcU | 3.093 | 9.69E-10 | 4.960 | 1.31E-20 | |
| PA14_RS05730 | PA3842 | spcS | SpcS | 6.434 | 1.11E-08 | 11.384 | 4.76E-15 |
E, exponential growth phase.
S, stationary growth phase.