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. 2016 Oct 13;36(21):2715–2727. doi: 10.1128/MCB.00138-16

FIG 1.

FIG 1

Creation of CD36Tg mice that overexpress CD36 in the liver. (A) Tet-off transgenic system to express CD36 in the liver. The open X indicates the silence of transgenic CD36 expression. (B and C) The mRNA expression of endogenous and transgenic CD36 (B) and transgenic CD36 (C) was detected by Northern blotting and real-time PCR, respectively. The Northern blotting probe detected both endogenous and transgenic CD36, whereas the real-time PCR specifically detected transgenic CD36. (D) Protein expression of the transgene and its silencing by Dox were measured by Western blotting. (E) Immunohistochemical staining of CD36. (F) Uptake of the fluorescent fatty acid analogue BODIPY-C16 by hepatocytes isolated from WT mice and CD36Tg mice treated or not treated with Dox. (G) Fluorometric quantification of BODIPY-C16 uptake. All the mice were maintained on a chow diet. *, P < 0.05; **, P < 0.01. The data are presented as means and SEM.