Table 2. Limits of quantification and detection for individual targets (in target copies per reaction) covered by the multiplex assays MTQ1 and MTQ2 calculated from fifteen replicates.
Sample | hmgA | MON863 | MON810 | DP98140 | MIR604 | GA21 | MON89034 | MIR162 |
---|---|---|---|---|---|---|---|---|
DNA mix 3 | 10079 | 121 | 176 | 175 | 123 | 105 | 134 | 131 |
DNA mix 4 | 3174 | 36a | 58 | 58 | 36a | 31a | 42a | 41a |
DNA mix 4a | 1536 | 17 | 26a | 29 | n.t. | n.t. | n.t. | n.t. |
DNA mix 4b | 1233 | 15 | 21 | 23a | n.t. | n.t. | n.t. | n.t. |
DNA mix 5 | 993 | 12b,c | 18b | 17b | 10b | 9b,c | 11b | 11b |
DNA mix 6 | 281 | neg | neg | neg | neg | neg | neg | neg |
DNA mix 7 | 96 | neg | neg | neg | neg | neg | neg | neg |
DNA mix 8 | 29a | neg | neg | neg | neg | neg | neg | neg |
DNA mix 9 | 10b,c | neg | neg | neg | neg | neg | neg | neg |
aLimit of quantification.
bLimit of detection.
cOne of fifteen replicates was negative with one or two positive droplets; neg – at least two replicates out of fifteen was negative.