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. 2016 Oct 14;6:35438. doi: 10.1038/srep35438

Figure 5. Effects of knockdown or overexpression of DGKζ or PIP5K1α on DGK or PIP5K activity.

Figure 5

3T3-L1 adipocytes were electroporated with non-relevant control siRNA (control), DGKζ siRNA, or PIP5K1α siRNA. Three days after electroporation, cell lysates were prepared to measure PIP5K activity (a) or DGK activity (b), respectively. Also, the CHO cells were transfected with mock vector or pFLAG-DGKζ plasmid, or pHA-PIP5K1α plasmid. Twenty four hours later, cell lysates were prepared to measure PIP5K activity (c) or DGK activity (d). CHO cells were transfected with mock vector or pGFP-sDGKζ plasmid for measuring the PIP5K and DGK activities (e,f). The methods to measure PIP5K or DGK activity were described in “Materials and Methods”. The results are presented at the means ± S.E.M. of three samples. These are representative data from experiments independently performed at least three times. The results are presented at the means ± S.E.M. of five samples. *p < 0.05 as compared with control group, #p < 0.05 as compared with mock group.