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. 2016 Oct 14;6:35166. doi: 10.1038/srep35166

Figure 3. Reprogramming of HCECs to progenitors by weekly but not by single knockdown with p120-Kaiso siRNAs.

Figure 3

HCECs received 5 weekly knockdowns since Day 7 or single knockdown at Day 42 with p120-Kaiso siRNAs or scRNA in MESCM. Immunostaining to Bmi1 and p16INK4a was correlated with BrdU labeling and immunostaining to Oct4, Sox2 and Nonog (A, Scale bars: 25 μm). Expression of neural crest markers analyzed at Day 42 was measured by qRT-PCR after a single knockdown at Day 42 (B) or weekly knockdowns from Day 7 till Day 42 (C, n = 3, *p < 0.05 and **p < 0.01, compared to scRNA control).