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. 2016 Oct 14;6:35166. doi: 10.1038/srep35166

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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HCECs received single knockdown at Day 21 (A,B) or 5 weekly knockdowns by scRNA or p120-Kaiso siRNAs since Day 7 (C,D) in MESCM. Expression of various cell cycle regulators was measured by qRT-PCR (A and C, *p < 0.05 and ^#p < 0.05 when compared to scRNA control). Cytolocalization of Bmi1 and p16^INK4a was assessed by immunostaining (B and D, Scale bars: 25 μm).