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. 2016 Oct 14;6:35166. doi: 10.1038/srep35166

Figure 5. Mitigation of reprogramming of HCECs to progenitors by p120-Kaiso knockdown by Bmi1 siRNA.

Figure 5

At Day 35, Bmi1 siRNA was added to HCECs receiving 5 weekly knockdowns with scRNA or p120-Kaiso siRNAs since Day 7. Transcript expression of p16INK4a (p16) (A) and various neural crest markers was compared by qRT-PCR (B, n = 3, #p < 0.05, *p < 0.05 and **p < 0.01 compared to scRNA control). Cytolocalization of p16INK4a, Oct4, Sox2, Nanog, and BrdU (red)/p120 (green) was also compared by immunostaining (C, Scale bars: 25 μm).