Figure 5. Antibody production in aAVC-OVA-immunized mice depends on CD4⁺ Tfh cells, but not iNKTfh cells.

(a,b) The generation of iNKTfh cells in WT or CD4^−/− aAVC-OVA-immunized and naïve mice. WT mice and CD4^−/− mice were immunized with aAVC-OVA. Five days later, the frequency of iNKTfh cells in spleen were analyzed using CD19-FITC, CD1d-dimer-PE, PD1-VB, and CXCR5-APC. (Mean ± SEM, n = 5–6) (c,d) The frequency of iNKTfh cells in spleen were analyzed in WT or MHC II^−/− mice 5 days after immunization with aAVC-OVA. (e) OVA-specific serum IgG1 and IgG2b antibody response were measured in WT mice and MHC II^−/− mice 2 weeks after immunization with aAVC-OVA. (Mean ± SEM, n = 5–6) *P < 0.05.