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. 2016 Oct 14;6:35101. doi: 10.1038/srep35101

Figure 1. Effect of TYLCSV, PSTVd, or double infection by TYLCSV/PSTVd on tomato plants.

Figure 1

(A) Symptoms on plants at 9 wpi. Non-infected plants were inoculated with pBIN19, as control. (B) Height and dry weight of plants at 11 wpi (12 plants per treatment). NI, non infected plants, T, infected by TYLCSV, P, infected by PSTVd, TP, co-infected by TYLCSV and PSTVd. Bars, standard errors; letters, statistical significance (P ≤ 0.05; Student t-test). (C) Accumulation of PSTVd in singly- or doubly-infected plants, simultaneously co-inoculated. (D,E) Accumulation of TYLCSV in singly-or doubly-infected plants, simultaneously co-inoculated. Agarose gels were run without (D) or with (E) ethidium bromide, the latter conditions allowing to separate the viral DNA forms. (F,G) Accumulation of TYLCSV in plants inoculated first with TYLCSV and superinfected with PSTVd or pBIN19, as control; gels run without (F) or with (G) ethidium bromide. Samples are always from plants at 6 weeks after TYLCSV inoculation. cc and lin, circular and linear PSTVd forms; rRNA, ribosomal RNA, shown as loading control; vDNA, TYLCSV DNA, including genomic and replicative forms; ss, genomic TYLCSV DNA; oc, sc, open circular and supercoiled TYLCSV dsDNA; gDNA, plant genomic DNA, shown as loading control.