Table 1. Oligonucleotides for real-time PCR.

Oligoa	Target	Sequence (5′–3′)	Modification	Lengthb	Tmc (°C)
Fw	PPIB	TGTGGTGTTTGGCAAAGT		278	57.7
Rev		TGGAATGTGAGGGGAGTG			58.4
Fw	Exon 6, CD46	TGACAGTAACAGTACTTGGGA			57.2
Rev1	Exon 12/13, CD46	ATCAGTTAGGTATGTGCCTTTC		289/244d	57.0
Rev2	Exon 12/14, CD46	ACCATCTGCTTTCCCTTTC		286/241d	56.7
Probe	BC, CD46	CCAAAGTGTCTTAAAGTGTCGACTTCTTCCACTAC	5′-ROXe3′-BHQ2	NRf	66.5
Probe	C, CD46	AGTGTCTTAAAGGTCCTAGGCCTACTTACAAGC	5′-ROXe3′-BHQ2	NR	66.9
Probe	PPIB	AGAGCACCAAGACAGACAGCCG	5′-FAM3′-BHQ1	NR	66.4
Comp	Exon 8/9, CD46	GTGCCTCAGGTCCTAGGCCTACTTAC	3′-phosphate	NR

^aOligo, oligonucleotides; indicated as Fw, forward primer; Rev, reverse primer; Probe, probe labeled with fluorescent dye and quencher; and Comp, complementary competitor oligonucleotide.

^bLength, bases of amplified product.

^cTm predicted by the program CLC Main Workbench 6.

^dLength of amplified BC/C isoforms using exon 6 forward primer.

^eROX analog CalFluor red 610.

^fNR, not relevant.