Skip to main content
. 2016 Oct 14;6:34962. doi: 10.1038/srep34962

Figure 2. Mediator, Cohesin and NIPBL are essential for maintenance of cancer cells properties.

Figure 2

(a) Heat map representation of KEGG pathways enriched for genes occupied by MED1, SMC1A and NIPBL in MCF7, HEPG2 and A549 cells (Fisher’s exact test, *p < 0.05, **p < 0.01, ***p < 0.001). (b) Growth curve of MCF7, HEPG2 and A549 following decreased expression of MED1, SMC1A and NIPBL showing a global negative effect on cell growth. Each line represents the average of a minimum of two biological replicates for two independent shRNA constructs. The p-values were calculated by comparing with the shRNA controls at day 8 (paired t-test, *p < 0.05 and **p < 0.01). (c) Soft agar colony formation assays with decreased levels of MED1, SMC1A and NIPBL showing a loss of colony formation potential. Colonies were quantified with the ImageJ software using 4 images per well. The bar graph represents the average of a minimum of two biological replicates for two independent shRNA constructs. The p-values were calculated by comparing with the shRNA controls (paired t-test, *p < 0.05 and **p < 0.01).