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F: forward primer, R: reverse primer.
| Primer name | Sequence (5′–3′) |
|---|---|
| XhoI-F | ccgctcgagGTGGTTGGAAGCCCAGCAGATGGGAG |
| NotI-R | ataagaatgcggccgcTAAGGTAAGCAATTATTTTATTTC |
| mut-F | TTTCACATGCTCAATTGATATTTTTACGACGATCCTCGGCCCAGGGAGAAAGCATGT |
| mut-R | ACATGCTTTCTCCCTGGGCCGAGGATCGTCGTAAAAATATCAATTGAGCATGTGAAA |
