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. 2016 Oct 14;7:1499. doi: 10.3389/fpls.2016.01499

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Copyright © 2016 Borowska-Zuchowska, Kwasniewski and Hasterok.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Structural organization of 35S rDNA intergenic spacers of B. hybridum (Bh IGS) and the modern relatives of ancestral species B. distachyon (Bd IGS) and B. stacei (Bs IGS). (A) PCR profiles of the intergenic spacers. The position of primers that were used for PCR is indicated below the diagram of the 18S-5.8S-25S rDNA unit. Bright bands in the marker line reflect 6 kb (upper band) and 3 kb (lower band). (B,C) Schematic representation of Bh IGS (B), Bd IGS (C) and Bs IGS (D). Repetitive sequences in each intergenic spacer are denoted as pentagons with different colors. Putative transcription initiation sites (TISs) are marked as arrows over the diagrams. (E–G) Dot matrix plots of the intergenic spacers. Self-comparisons of Bh IGS (E), Bd IGS (F), and Bs IGS (G). Position of the repetitive sequences is indicated by rectangles.