Figure 7.

Schematic representation of host cell invasion by T. cruzi MT and TCT. Binding of MT to host cell, mediated by gp82, triggers lysosome mobilization to the cell periphery. Gp82 induces dephosphrylation of mTOR associated with TFEB at the lysosome surface, TFEB translocation to the nucleus and lysosome biogenesis. TCT invasion is predominantly lysosome‐independent. Conditions that stimulate lysosome biogenesis and/or scattering, such as short term incubation in nutrient‐deprived PBS⁺⁺ solution or treatment with sucrose, increase MT invasion and greatly reduce TCT internalization. Conversely, lysosome retention at the perinuclear region, upon treatment with mTOR inhibitor rapamycin, reduces MT invasion and facilitate TCT internalization.