Table 1.
SEMA4D Saturation Assay—Intra‐assay Imprecision.
| Reportable | Lowa | Midb | Highc | Mean | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Result | Mean | SD | %CV | Mean | SD | %CV | Mean | SD | %CV | %CV |
| Percent Saturation | 11.0 | 0.578 | 5.24 | 36.3 | 1.40 | 3.84 | 98.2 | 7.11 | 7.24 | 5.44 |
| VX15/2503 FOI | 203 | 19.7 | 9.68 | 246 | 5.60 | 2.27 | 34.2 | 3.45 | 10.1 | 7.35 |
| mAb 2282‐biotin FOI | 96.7 | 4.40 | 4.55 | 112 | 3.04 | 2.71 | 22.6 | 1.65 | 7.30 | 4.85 |
Whole blood collected from three apparently healthy volunteers was dosed ex vivo with varying concentrations of VX15/2503. Samples were then assayed in triplicate in a single analytical run.
a
Donor 3 spiked with approximately 10 ng/mL VX15/2503.
b
Donor 2 spiked with approximately 50 ng/mL VX15/2503.
c
Donor 1 spiked with approximately 10 μg/mL VX15/2503.