Figure 6.

Mrap2^tm1a/tm1a mice exhibit fatty liver and changes in cholesterol metabolism. Morphological changes in the liver of 129/Sv Mrap2^tm1a/tm1a mutant mice as shown by H&E staining (A) and Oil Red O (B), suggesting lipid accumulation in Mrap2^tm1a/tm1a. Scale bars=200μm. (C, D, E, F, G and H) Circulating TAG, total cholesterol, HDL, LDL, NEFA-C and Glycerol in 16-week C57BL/6N Mrap2^tm1a/tm1a mice are shown, n=7 for each Mrap2^tm1a/tm1a group, 38 for female Mrap2^+/+ and 35 for Mrap2^+/+ controls gender/genotype. The P-values presented on graphs are either global P-values for genotype adjusted for multiple correction testing, or (in the cases of sexual dimorphism) the P-value is the impact of genotype for that sex. NS, not significant. *P<0.05; **P<0.005; ***P<0.0005; NS, not significant. (I) Elevated expression levels of Srebp2 in Mrap2^tm1a/tm1a female mice, reduced Ldlr in male Mrap2^tm1a/tm1a mice and expression levels of Scarb1 in female mice and male mice (n=4 for each genotype/gender, *P<0.05, NS, not significant).