Figure 3.

Effects of embryonic treatments on movement, tactile responses, swimming and cortisol levels. Results show the effects of dexamethasone, GR morpholino knockdown (GR Mo) and hypoxia treatment on: (A) spontaneous twitch movement at 48h post fertilisation (hpf (n=3 experiments, 20 larvae per group)); (B) tactile responses of 96hpf larvae (n=5 experiments, 10 larvae per experiment); (C) Swim distance (mm/min) and (D) velocity during active swimming (mm/s) of 120hpf larvae averaged over a 5min period of observation (n=12 larvae); (E) developmental changes in whole larvae cortisol from 72 to 120hpf (n=3 experiments, 15 larvae pooled/group). Values are mean±s.e.m. and were analysed by one-way ANOVA and Bonferroni post hoc analysis.