Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2016 Oct 14;16:241. doi: 10.1186/s12866-016-0852-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Characterization of the expressed and purified chimeric r4R protein. a SDS-PAGE analysis of the expression and purification steps of the r4R protein. Pellet (lane 1 and 3) and supernatant (lane 2 and 4) fractions of lysates from E. coli BL21 (DE3) cells containing the empty vector pET28a (lane 1 and 2) or pET28a-4R (lane 3 and 4) were electrophoresed in a 10 % SDS-PAGE gel. M indicates the protein ladder. Lane 5 contains the purified recombinant 4R protein. b Anti-Leptospira Western blot analysis. The purified chimeric r4R protein was run on a SDS-PAGE gel and transferred to a PVDF membrane. Serum from heat-killed L. interrogans strain Lai immunized rabbits was used as a primary antibody to detect r4R. Serum from PBS injected rabbits was used as control. c Anti-r4R Western analysis. The purified chimeric r4R protein was run on SDS-PAGE gel and transferred to a PVDF membrane. Serum from guinea pigsimmunized with PBS (negative control) or chimeric protein, were used as primary antibody