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. 2016 Oct 14;16:241. doi: 10.1186/s12866-016-0852-y

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Proliferative and immune responses of splenocytes immunized with chimeric protein. Guinea pigs were immunized with the chimeric r4R protein. Ten days after the last immunization, the splenocytes were isolated, stimulated with the r4R protein and cultured for 72 h. a Splenocyte proliferation analysis of PBS, ConA and r4R stimulated splenocytes. b ELISA measurements of IL-4 and INF-γ secreted by pooled control, ConA and r4R stimulated splenocytes and lymphocytes in the culture supernatants