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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Ann Biomed Eng. 2016 Apr 11;45(2):439–451. doi: 10.1007/s10439-016-1605-7

Figure 5.

Figure 5

Reconstructed cross-sectional images of the explanted valves (a, d, g) with the proximal side on the left and the distal side on the right. (a) Trichrome staining revealed collagen (green) throughout the entire valve (b) leaflet and (c) root. (d) Von Kossa staining revealed no apparent calcification in the valve (e) leaflet and (f) root, except near some of the degradable sutures where calcific nodules formed in three animals. Arrow points to calcification present near anastomosis in the pulmonary artery (g) Verhoeff staining to detect mature elastin (black) in the valve (h) leaflets and (i) root. Scale bars for all images are 250 μm.