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. Author manuscript; available in PMC: 2017 Oct 4.
Published in final edited form as: Structure. 2016 Sep 15;24(10):1830–1841. doi: 10.1016/j.str.2016.07.017

Figure 3.

Figure 3

Effect of detergent on ND bilayer. LspA containing dH5 DMPC ND titrated with diC7PC as monitored by 31P NMR at 202 MHz. The highfield and lowfield signals are assigned to DMPC and diC7PC, respectively. The DMPC signals exhibit a splitting at detergent-lipid ratios between approximately 0.6 and 1.0, what might be interpreted as a decrease in particle size from ND to smaller bilayers. Lipid:detergent ratios were calculated from the integrated peak volumes when peaks are resolved. For conditions without resolved peaks the ratios were back-calculated based on the amount of detergent added.