Table 1. Combinatorial selective labelling scheme employed for backbone assignment of LspA.
labelling type a | sample # | ||
---|---|---|---|
|
|||
1 | 2 | 3 | |
2H/15N | Thr | Thr | Thr |
Lys | Lys | Phe | |
Trp | Tyr | Trp | |
Leu | Arg | Arg | |
2H/13C/15N b | Ala | Ile | Val |
1-13C c | Pro | Pro | Ser |
Asp | Ser | Asp | |
2-13C d | Phe | Phe | Leu |
Tyr | Leu | Tyr |
amino acids not listed were uniformly 2H labelled.
fully 13C and 15N labelled.
selectively 13C labelled at the carbonyl position.
selectively 13C-labeled at the α-position.