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. Author manuscript; available in PMC: 2017 Mar 5.
Published in final edited form as: Nat Biotechnol. 2016 Sep 5;34(10):1052–1059. doi: 10.1038/nbt.3666

Figure 3. Measuring single immune cell growth patterns and activation dynamics.

Figure 3

(A) Mouse CD8+ T cells activated with anti-CD3 and anti-CD28 show an initial increase in the growing fraction between 24 and 48 hours, followed by a consistent mass-growth profile over the next three days. Error bars are standard errors of the slope, estimated directly from the regression fit. Points are overlaid on corresponding kernel density estimates (blue). (B) Measuring lymphocyte activation by quantifying mass and growth of single human PBMCs. After the initial loss of a presumed monocyte subpopulation between 20–30 pg (likely due to adherence to the culture well), we observed the emergence of a subpopulation containing larger and correspondingly faster-growing cells.