Figure 6. ROS levels in young and old mice oocytes after treatment with H₂O₂.

A. ROS levels are significantly elevated in old mice germinal vesicle stage oocytes following H₂O₂ treatment. B. ROS levels are significantly elevated in old mice MII oocytes after treatment with H₂O₂. Fluorescence intensity of Carboxy-H2DCFDA was used to measure ROS levels. Asterisks indicate statistical significance. Unpaired t-test was used for statistical analysis.

Abbreviations: ROS, reactive oxygen species; H₂O₂: hydrogen peroxide; carboxy-H2DCFDA: 6-carboxy-2',7'-dichlorodihydrofluorescein diacetate.