Figure 5.

The Effect of Idh1^R132H on Neurogenesis and Astrocyte Genesis in the Olfactory Bulb

(A) BrdU, Dcx, and NeuN expressing cells (neurogenesis) are shown in representative images from Tam-Idh1-KI (n = 4) and control (n = 4) OBs. The left-hand image at the bottom of each panel is a magnified orthogonal 3D view of the BrdU⁺Dcx⁻NeuN^- cell marked by a white arrow; the adjacent middle image is the equivalent view of the BrdU⁺Dcx⁺NeuN⁻ cell marked by a white arrowhead; and the adjacent right-hand image is the equivalent view of the BrdU⁺Dcx⁻NeuN⁺ cell marked by a yellow arrow. RMS, rostral migratory stream; GCL, granule cell layer; IPL, internal plexiform layer; ML, mitral layer; EPL, external plexiform layer. The cell counts are quantitated in the bar chart.

(B) BrdU, GFAP, and Olig2 (astrocyte genesis) data are shown from the same mice as in (A). The left-hand image at the bottom of each panel is a magnified orthogonal 3D view of the BrdU⁺GFAP⁺ cell marked by a white arrow; and the adjacent right-hand image is the equivalent view of the BrdU⁺Dcx⁻NeuN⁺ cell marked by a white arrowhead. Other annotations are as per (A).

(C) A schematic of the multi-layered cellular architecture of a mouse OB is shown. GL, glomerular layer. Other annotations are as per (A).

All data are presented as mean ± SD (^∗p < 0.05, ^∗∗∗p < 0.005). See also Figure S4.